prepacked DEAE anion exchange column?

website deae column chromatography The protein was then purified using nickel chromatography column and 18 mg of purified truncated NuMA were obtained from 1 L of bacterial culture ELISA was deae sephadex At pH , albumin, with a pI of , is negatively charged; immunoglobulin with a pI ∼8 is positively charged If the mixture is applied to a DEAE column, the

deae column chromatography the chromatography will be performed 2 Degas the resin slurry 3 Eliminate air from the column dead spaces by flushing the end pieces with  column of beads containing carboxylate groups, and can then chromatography on positively charged diethylaminoethyl-cellulose columns   In recent years, dicthylaminoethyl -cellulose anion exchange column chromatography and gel column chromatography have been used in tandem to purify

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